Cell Proliferation High-Content Screening on Adherent Cell Cultures
Related Experiment Video
Updated: Jan 20, 2026

Using High Content Imaging to Quantify Target Engagement in Adherent Cells
Published on: November 29, 2018
Pau Carrillo-Barberà1,2,3, Jose M Morante-Redolat1,2,3, José F Pertusa4
1Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, Burjassot, Spain.
Abstract:
Pulse-chase experiments using 5-bromo-2'-deoxyuridine (BrdU), or the more recent EdU (5-etynil-2'-deoxyuridine), enable the identification of cells going through S phase. This chapter describes a high-content proliferation assay pipeline for adherent cell cultures. High-throughput imaging is followed by high-content data analysis using a non-supervised ImageJ macroinstruction that segments the individual nuclei, determines the nucleoside analogue absence/presence, and measures the signal of up to two additional nuclear markers. Based upon the specific combination with proliferation-specific protein immunostaining, the percentage of cells undergoing different phases of the cell cycle (G0, G1, S, G2, and M) might be established. The method can be also used to estimate the proliferation (S phase) rate of particular cell subpopulations identified through labelling with specific nuclear markers.
Related Concept Videos
Cells Coordinate Growth and Proliferation
Cell Culture
Stem Cell Culture
Abnormal Proliferation
Stereotype Content Model
Measurement of Air Content in Concrete
The pressure method,...

