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Related Concept Videos

Mechanism of Filopodia Formation01:39

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Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
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When drugs are administered, they can elicit either an agonist or antagonist effect on the body. Agonism occurs when a drug activates a specific receptor, triggering a biological response. On the other hand, antagonism happens when a drug binds to the same receptors but blocks their activation, thereby preventing a biological response.
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Purification of the Dendritic Filopodia-rich Fraction
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Filopodia Quantification Using FiloQuant.

Guillaume Jacquemet1,2, Hellyeh Hamidi3, Johanna Ivaska3

  • 1Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland. Guillaume.jacquemet@utu.fi.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2019
PubMed
Summary
This summary is machine-generated.

FiloQuant is a new open-access software for quantifying filopodia length, density, and dynamics. This tool aids in understanding cell protrusions in biological processes and cancer progression.

Keywords:
FijiFiloQuantFilopodiaFilopodia propertiesFilopodia-like protrusionsImageJ

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Area of Science:

  • Cell Biology
  • Biophysics
  • Bioinformatics

Background:

  • Filopodia are crucial cell protrusions involved in sensing the environment.
  • They play roles in development, wound healing, and cancer progression.
  • Analyzing filopodia requires specialized software for mechanistic insights.

Purpose of the Study:

  • To introduce FiloQuant, an open-access software for quantifying filopodia.
  • To provide protocols for installing and using FiloQuant within ImageJ (Fiji).
  • To demonstrate filopodia quantification from static and live-cell imaging.

Main Methods:

  • FiloQuant software installation on ImageJ (Fiji).
  • Quantification of filopodia length and density from single images.
  • Tracking filopodial dynamics using FiloQuant and TrackMate in live-cell imaging.

Main Results:

  • Successful installation and application of FiloQuant for filopodia analysis.
  • Accurate quantification of filopodia length, density, and dynamics.
  • Demonstrated utility in both in vitro and in vivo samples.

Conclusions:

  • FiloQuant provides a robust, open-access solution for filopodia analysis.
  • The software facilitates mechanistic studies of filopodia in various biological contexts.
  • FiloQuant enhances the study of cell protrusions in development and disease.