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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
Published on: May 29, 2020
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High-throughput multiplexed autoantibody detection to screen type 1 diabetes and multiple autoimmune diseases
Yong Gu1, Zhiyuan Zhao2, Kathleen Waugh2
1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, Aurora, CO, United States of America; Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University, China.
Ebiomedicine
|August 27, 2019
Summary
A new multiplexed assay simultaneously screens for type 1 diabetes (T1D) and other autoimmune diseases. This electrochemiluminescence (ECL) assay is sensitive, specific, high-throughput, and cost-effective for large-scale population screening.
Area of Science:
- Immunology
- Biochemistry
- Clinical Diagnostics
Background:
- Islet autoantibodies (IAbs) are key biomarkers for type 1 diabetes (T1D) risk.
- Current single autoantibody radio-binding assays (RBAs) are inefficient for large-scale screening.
- Co-occurring autoimmune conditions in T1D patients necessitate simultaneous screening.
Purpose of the Study:
- To develop a multiplexed assay for simultaneous screening of T1D and other autoimmune diseases.
- To validate a novel electrochemiluminescence (ECL) assay platform for multiplexed autoantibody detection.
Main Methods:
- Developed a 7-plex electrochemiluminescence (ECL) assay combining 7 autoantibody tests in a single well.
- Validated the 7-plex ECL assay against standard radio-binding assays (RBAs) and single ECL assays.
Main Results:
- The 7-plex ECL assay demonstrated strong correlation with individual autoantibody assays (ECL and RBA).
- The assay effectively screens for T1D, celiac disease, autoimmune thyroid disease, and APS-1.
Conclusions:
- The multiplexed ECL assay offers high sensitivity and specificity for T1D and related autoimmune diseases.
- This high-throughput, cost-effective assay is suitable for large-scale population screening.
- Enables simultaneous detection of multiple critical autoantibodies.
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