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Updated: Jan 20, 2026

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
Published on: May 26, 2016
In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous
Nigel Yarlett1,2, Mary Morada3, Mohini Gobin3
1Haskins Laboratories, Pace University, New York, NY, USA. nyarlett@pace.edu.
Abstract:
Hollow fiber technology is a powerful tool for the culture of difficult-to-grow cells. Cryptosporidium parvum has a multistage sexual and asexual life cycle that has proved difficult to culture by conventional in vitro culture methods. Here, we describe a method utilizing a hollow fiber bioreactor for the continuous in vitro growth of C. parvum that produces sexual and asexual stages. The method enables the evaluation of potential therapeutic compounds under conditions that mirror the dynamic conditions found in the gut facilitating preliminary pharmacokinetic and pharmacodynamic data to be obtained.
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