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Lymphokine production by human peripheral blood lymphocytes: analysis by in situ hybridization
G Qiu1, J F Gauchat, U Wirthmüller
1Institute of Clinical Immunology, Inselspital, University of Bern, Switzerland.
Lymphokine Research
|January 1, 1988
Abstract:
Lymphokine production in human peripheral blood mononuclear cells (PBMNCs) was investigated by in situ hybridization with 35S-labelled RNA probes coding for interleukin 2 (IL-2), gamma-interferon (gamma-IFN) and granulocyte/macrophage colony stimulating factor (GM-CSF). The proportion of PBMNCs expressing lymphokine mRNA varied according to the stimuli used and followed similar patterns to those previously observed by Northern blot analysis. Only a relatively low proportion of PBMNCs expressed lymphokine mRNA after stimulation for a given time, and this seems not to be due to the heterogeneity of T cells.