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New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction

C Bergna, G H Marin1, M Maiz

  • 1Faculty of Medical Science, National University of La Plata, Argentina. gmarin2009@gmail.com

The Ceylon Medical Journal
|August 29, 2019
PubMed
Abstract

Insights

An experimental peptide significantly enhanced apoptosis and survival in mice with aggressive lymphoma when combined with cyclophosphamide (CFM). This peptide potentiates CFM

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • The RAS-RAF-MEK-ERK pathway is crucial for regulating apoptosis in cancer cells.
  • B-Raf inhibitors, like the PLX4032 peptide, are developed to induce apoptosis in cancer cells.

Purpose of the Study:

  • To evaluate the pro-apoptotic properties and survival outcomes of the EP2014/064243 peptide in a murine model of aggressive lymphoma.
  • To assess the combined effect of the peptide and cyclophosphamide (CFM) on lymphoma progression.

Main Methods:

  • BALB/c mice with T-lymphoma were randomized into four groups: peptide + CFM, peptide alone, CFM alone, and saline control.
  • Survival probability was analyzed using Kaplan-Meier analysis.
  • Apoptosis was quantified using the TUNEL assay.

Main Results:

  • The combination of EP2014/064243 peptide and CFM significantly increased median survival compared to CFM monotherapy (34 vs. 33 days).
  • The peptide alone also showed a survival benefit (33 days) compared to the control group.
  • TUNEL assay revealed a significantly higher number of apoptotic cells in lymph node tissue for the peptide and combined treatment groups compared to CFM alone and control.

Conclusions:

  • The experimental peptide demonstrates significant pro-apoptotic effects in murine aggressive lymphoma.
  • Combining the peptide with cyclophosphamide (CFM) potentiates the anti-tumoural effect, leading to improved survival outcomes.
  • The peptide enhances the efficacy of CFM, suggesting a synergistic therapeutic potential.
Keywords:
T-LYMPHOMANHL-TPeptidesApoptosis

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