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Related Concept Videos

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

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Nuclear magnetic resonance (NMR) spectroscopy is used to identify dysregulation in the metabolites in patients with various diseases. This technique allows the quantification of the deranged metabolites, unraveling the pathophysiological insights. Here, we describe the step-by-step procedure of the NMR-based approach for the metabolic characterization of the patients.
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Applications Of NMR In Biology01:25

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are...
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A simple and non-destructive technique that measures the average content of drug substances in formulated drug products containing fluorine using low-field fluorine-19 (19F) time-domain (TD) nuclear magnetic resonance (NMR) is presented here. The technique can be applied to the development and manufacturing of drugs in the pharmaceutical...
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Related Experiment Video

Updated: Jan 20, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

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NMR-Based Urinary Metabolomics Applications.

Tedros Bezabeh1, Ana Capati2,3, Omkar B Ijare4,5

  • 1College of Natural and Applied Sciences, University of Guam, Mangilao, GU, USA. bezabeht@triton.uog.edu.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Summary

Nuclear Magnetic Resonance (NMR)-based urinary metabolomics shows promise for biomedical applications. Optimization and standardization of protocols are crucial for its clinical acceptance and widespread use.

Keywords:
Metabolic profileMetabolomicsMultivariate analysisNMR spectroscopySpectral profileUrinalysisUrine

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Last Updated: Jan 20, 2026

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Area of Science:

  • Metabolomics
  • Biomedical Science
  • Analytical Chemistry

Background:

  • Metabolomics is a rapidly expanding field with growing interest in nuclear magnetic resonance (NMR)-based urinary metabolomics.
  • NMR-based urinary metabolomics has demonstrated potential in various applications, but requires further development for clinical adoption.

Purpose of the Study:

  • To present recommended methods and procedures for NMR-based urinary metabolomics.
  • To highlight challenges and propose solutions for advancing NMR-based urinary metabolomics in biomedical applications.

Main Methods:

  • Review and recommendation of optimized parameters for NMR-based urinary metabolomics.
  • Discussion of protocol standardization for improved reproducibility and reliability.

Main Results:

  • Identified key areas for methodological optimization in urinary metabolomics.
  • Outlined strategies to overcome existing challenges in the application of NMR-based metabolomics.

Conclusions:

  • Standardization and optimization are essential for the clinical acceptance of NMR-based urinary metabolomics.
  • Addressing current challenges will facilitate the broader application of this technique in diagnostics and biomedical research.