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Related Experiment Video

Updated: Jan 20, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

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Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization.

Mathilde H Lerche1, Magnus Karlsson1, Jan H Ardenkjær-Larsen2

  • 1Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a method for analyzing metabolite profiles using hyperpolarized 13C-NMR. This technique enhances sensitivity for metabolic studies and provides insights into living cells.

Keywords:
13C-NMRGlucoseHyperpolarizationdDNP

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Area of Science:

  • Biochemistry
  • Metabolomics
  • Nuclear Magnetic Resonance (NMR) Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) is a noninvasive technique for studying metabolite profiles in complex mixtures.
  • Hyperpolarized 13C-NMR spectroscopy, enhanced by quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment, significantly increases sensitivity for metabolic analyses.
  • This improved sensitivity allows for detailed mapping and quantification of metabolic pathways and networks.

Purpose of the Study:

  • To describe a reproducible and quantitative sample preparation method for analyzing hyperpolarized 13C-NMR metabolite spectra.
  • To demonstrate the application of qdDNP for gaining metabolic insights into living cells.

Main Methods:

  • Development of a sample preparation protocol involving cell incubation, extraction, and signal enhancement.
  • Utilizing quantitative dissolution Dynamic Nuclear Polarization (qdDNP) for hyperpolarization of 13C-labeled metabolites.
  • Acquisition and analysis of hyperpolarized 13C-NMR spectra.

Main Results:

  • A robust method for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra was established.
  • The method enables enhanced sensitivity for detecting and quantifying metabolites.
  • Demonstrated the utility of qdDNP for metabolic profiling of living cells.

Conclusions:

  • The described sample preparation method facilitates quantitative analysis of hyperpolarized 13C-NMR spectra.
  • Hyperpolarized 13C-NMR with qdDNP is a powerful tool for noninvasive metabolic studies.
  • This approach provides valuable insights into metabolic pathways and networks in living systems.