Related Experiment Video
Updated: Jan 20, 2026

Alizarin Red Staining: A Technique to Visualize Mineralization by Cultured Osteoblasts
Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton
1Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA. Meadt2@ccf.org.
Abstract:
Understanding proteolytic remodeling of extracellular matrix involves the generation of global or conditional knockout mice by homologous recombination in embryonic stem cells or their manipulation through new advanced technologies such as CRISPR-Cas9. These models provide opportunities to understand the roles of ADAMTS genes in skeletogenesis. Whole-mount skeletal preparations are necessary for assessment of the skeletal phenotype. They allow for facile visualization of skeletal patterning, size and shape of skeletal elements, and skeletal structure. This protocol describes the staining of the murine skeleton using Alcian blue to identify cartilage and alizarin red to identify bone.
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