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Updated: Jan 20, 2026

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
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Visualization and Quantification of Pericellular Matrix.

Timothy J Mead1, Suneel S Apte2

  • 1Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

The pericellular matrix (PCM), a cell layer, influences cell function. Visualizing PCM helps understand its role in cell processes and remodeling by ADAMTS proteases.

Keywords:
ErythrocyteGlycocalyxParticle exclusionPericellular matrixRed blood cell

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Extracellular Matrix Research

Background:

  • The pericellular matrix (PCM), also termed glycocalyx, is situated between the cell membrane and the interstitial extracellular matrix (ECM).
  • PCM significantly impacts cellular functions due to its position at the cell-microenvironment interface.

Purpose of the Study:

  • To highlight the importance of PCM visualization and quantification.
  • To explore the role of ADAMTS proteases in PCM remodeling across various cell types and biological processes.

Main Methods:

  • Utilizing the particle exclusion assay for PCM demonstration.
  • Employing fixed red blood cells to delineate the cell boundary and associated PCM.

Main Results:

  • Established PCM visualization and quantification as valuable techniques.
  • Provided a foundation for investigating ADAMTS proteases' function in PCM remodeling.

Conclusions:

  • PCM visualization is crucial for understanding cell-environment interactions.
  • ADAMTS proteases are key players in modulating the PCM, impacting cellular behavior and function.