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Related Concept Videos

Introduction to Innate and Adaptive Immunity01:21

Introduction to Innate and Adaptive Immunity

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The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
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Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

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The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages12:47

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

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In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine...
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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

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We describe a protocol to measure the antiviral cytokine production in mice infected with a model herpesvirus, murine gamma herpesvirus 68 (γHV68) that is closely-related to human Kaposi’s sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV). Utilizing genetically modified mouse strains and mouse embryonic fibroblasts (MEFs), we assessed the antiviral cytokine production both in vivo and ex vivo. “Reconstituting” the expression of innate immune components in...
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Evaluation of Caspase Activation to Assess Innate Immune Cell Death10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

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This protocol describes a comprehensive method for assessing caspase activation (caspase-1, caspase-3, caspase-7, caspase-8, caspase-9, and caspase-11) in response to both in vitro and in vivo (in mice) models of infection, sterile insults, and cancer to determine the initiation of cell death pathways, such as pyroptosis, apoptosis, necroptosis, and...
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Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish07:07

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

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The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically...
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Related Experiment Video

Updated: Jan 20, 2026

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
12:47

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

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Innate Immunity.

Bani Preet Kaur1, Elizabeth Secord2

  • 1Detroit Medical Center, Children's Hospital of Michigan, 3901 Beaubien Boulevard, Detroit, MI 48201, USA.

Pediatric Clinics of North America
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

The innate immune system defends the body using specific receptors that trigger rapid inflammation and activate adaptive immunity. Understanding this system enables new therapeutic strategies through immune response modulation.

Keywords:
Anatomic barriersImmune modulationInflammatory responseInnate immunityMicrobial defensePhysiologic barriers

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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
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Evaluation of Caspase Activation to Assess Innate Immune Cell Death
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Related Experiment Videos

Last Updated: Jan 20, 2026

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

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Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

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Evaluation of Caspase Activation to Assess Innate Immune Cell Death
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Area of Science:

  • Immunology
  • Microbiology
  • Inflammation Research

Background:

  • The innate immune system is a crucial first line of defense against pathogens.
  • It utilizes germline-encoded receptors to recognize conserved microbial molecules.
  • This recognition initiates rapid inflammatory responses and primes adaptive immunity.

Purpose of the Study:

  • To summarize the role of the innate immune system in host defense.
  • To highlight the mechanisms by which innate immunity activates adaptive immunity.
  • To discuss the therapeutic potential of modulating innate immune responses.

Main Methods:

  • Review of current literature on innate immunity.
  • Analysis of receptor-ligand interactions in microbial recognition.
  • Exploration of immunomodulatory therapeutic approaches.

Main Results:

  • Innate immune receptors provide rapid, non-specific defense.
  • Activation of innate immunity is essential for initiating adaptive immune responses.
  • Targeting innate immune pathways offers promising therapeutic avenues.

Conclusions:

  • The innate immune system is fundamental to host defense and adaptive immunity.
  • Further research into innate immune mechanisms can yield novel immunotherapies.
  • Modulation of immune responses holds significant therapeutic potential for various diseases.