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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Phase Contrast and Differential Interference Contrast (DIC) Microscopy06:49

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

This protocol highlights the principles and practical applications of Phase and Differential Interference Contrast (DIC)...
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Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)09:38

Updated Protocol for the Assembly and Use of the Minibioreactor Array (MBRA)

The Minibioreactor Array (MBRA) is a high-throughput, customizable, continuous-flow culture system that enables the cultivation of complex microbial communities, supporting parallel experiments to study microbiome dynamics, therapeutic interactions, and microbial responses to environmental...
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Microbial Growth Media01:27

Microbial Growth Media

Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
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Contrast Enhanced Vessel Imaging using MicroCT05:50

Contrast Enhanced Vessel Imaging using MicroCT

Contrast enhanced small animal vessel imaging by microCT is a rapid, cost-effective and high-throughput technique for serial in situ examination for tumor development, for analyzing the network of blood vessels that nourish them, and for following the response of tumors to preclinical therapeutic...
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Manual Segmentation of the Human Choroid Plexus Using Brain MRI04:25

Manual Segmentation of the Human Choroid Plexus Using Brain MRI

Despite the crucial role of the choroid plexus in the brain, neuroimaging studies of this structure are scarce due to the lack of reliable automated segmentation tools. The present protocol aims to ensure gold-standard manual segmentation of the choroid plexus that can inform future neuroimaging studies.
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