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Related Concept Videos

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence09:11

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The micronucleus (MN) assay is a well-established test for quantifying DNA damage. However, scoring the assay using conventional techniques such as manual microscopy or feature-based image analysis is laborious and challenging. This paper describes the methodology to develop an artificial intelligence model to score the MN assay using imaging flow cytometry...
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The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay using manual microscopy is laborious and suffers from subjectivity and inter-scorer variability. This paper describes the protocol developed to perform a fully automated version of the assay using multispectral imaging flow...
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Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3
1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France
2 INSERM U1223, Paris, France
3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France
4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France
The cell cycle is a universal process of life. During the cell cycle, a cell...
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Related Experiment Video

Updated: Jan 20, 2026

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

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Micronucleus Analysis by Flow Cytometry.

Azeddine Elhajouji1, Pascal Stadelmann2

  • 1Genetic Toxicology and Photosafety, Preclinical Safety, Novartis Institutes for Biomedical Research, Basel, Switzerland. Azeddine.elhajouji@novartis.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2019
PubMed
Summary

The micronucleus (MN) test is a versatile biomarker for chromosomal damage, widely used for genotoxicity screening. This chapter details MN test protocols and flow cytometry analysis for accurate results.

Keywords:
Flow cytometryGenotoxicityHuman lymphocytesIn vitroIn vivoMicronucleus testRodents

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Area of Science:

  • Toxicology
  • Genetics
  • Biomarkers

Background:

  • The micronucleus (MN) test is a crucial biomarker for detecting chromosomal damage and mitotic abnormalities.
  • It originates from chromosome fragments or whole chromosomes mis-segregated during cell division.
  • Its accuracy, automation potential, and applicability to various cell types have made it invaluable in toxicology.

Purpose of the Study:

  • To provide an overview of protocol designs for performing the micronucleus (MN) test.
  • To detail experimental steps for successful MN test execution.
  • To explain accurate analysis of MN using flow cytometry.

Main Methods:

  • Micronucleus (MN) test protocol design.
  • In vitro and in vivo applications of the MN assay.
  • Flow cytometry for micronucleus analysis.

Main Results:

  • The MN test is a widely adopted genotoxicity screening tool.
  • OECD guidelines (487 for in vitro, 474 for in vivo) support its regulatory use.
  • Flow cytometry offers an accurate method for MN analysis.

Conclusions:

  • The MN test is a versatile and essential tool in genotoxicity assessment.
  • Standardized protocols and advanced techniques like flow cytometry enhance its utility.
  • The assay plays a key role in both research and regulatory toxicology.