Related Experiment Video
Updated: Jan 20, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
A Robust and Universal Metaproteomics Workflow for Research Studies and Routine Diagnostics Within 24 h Using Phenol
Robert Heyer1, Kay Schallert1, Anja Büdel1
1Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany.
Abstract:
The investigation of microbial proteins by mass spectrometry (metaproteomics) is a key technology for simultaneously assessing the taxonomic composition and the functionality of microbial communities in medical, environmental, and biotechnological applications. We present an improved metaproteomics workflow using an updated sample preparation and a new version of the MetaProteomeAnalyzer software for data analysis. High resolution by multidimensional separation (GeLC, MudPIT) was sacrificed to aim at fast analysis of a broad range of different samples in less than 24 h. The improved workflow generated at least two times as many protein identifications than our previous workflow, and a drastic increase of taxonomic and functional annotations. Improvements of all aspects of the workflow, particularly the speed, are first steps toward potential routine clinical diagnostics (i.e., fecal samples) and analysis of technical and environmental samples. The MetaProteomeAnalyzer is provided to the scientific community as a central remote server solution at www.mpa.ovgu.de.
More Related Videos
09:52A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
Published on: January 10, 2025
06:08From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
Published on: July 22, 2021
Related Concept Videos
14:48Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
09:52A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
06:08From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow
09:18Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow
07:05Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
13:43A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion