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Updated: Jan 20, 2026

Colorimetric Assay for Quantification of Lactate in Test Samples
Lactate Equivalent for Maximal Lactate Steady State Determination in Soccer
Ibai Garcia-Tabar1,2, Ermanno Rampinini3, Esteban M Gorostiaga1
1Government of Navarre.
The Minimum Lactate Equivalent (LEmin) effectively predicts Maximal Lactate Steady State (MLSS) in athletes. This study provides a practical method for estimating MLSS using a single submaximal test.
Area of Science:
- Exercise Physiology
- Sports Science
- Biochemistry
Background:
- Maximal Lactate Steady State (MLSS) is a key indicator of endurance performance.
- Predicting MLSS typically requires multiple, time-consuming tests.
- The Minimum Lactate Equivalent (LEmin) is a less-explored lactate threshold.
Purpose of the Study:
- To evaluate the Minimum Lactate Equivalent (LEmin) for predicting Maximal Lactate Steady State (MLSS) in soccer players.
- To compare LEmin's predictive accuracy against established blood lactate thresholds (BLTs).
- To assess LEmin's applicability in individuals with lower aerobic conditioning.
Main Methods:
- Fifteen soccer players performed a submaximal incremental running test to determine BLTs.
- MLSS was determined through 3-6 constant velocity running tests.
- LEmin was calculated and compared to conventional LTs and MLSS.
Main Results:
- LEmin showed no significant difference from conventional lactate thresholds (LTs).
- LEmin accurately predicted MLSS velocity (MLSSv) in soccer players (r=0.83).
- Combined data showed LEmin as a strong predictor of MLSSv (r=0.92).
Conclusions:
- LEmin is a highly effective single predictor of MLSS.
- This study offers practical regression equations for estimating MLSS from LEmin.
- LEmin provides a feasible alternative for assessing MLSS in soccer players.
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