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Related Concept Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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We clone and analyze reporter genes generating circular RNAs. These reporter genes are larger than constructs to analyze linear splicing and contain Alu elements. To investigate the circular RNAs, the constructs are transfected into cells and resulting RNA is analyzed using RT-PCR after removal of linear...
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Quantification of Circular RNAs Using Digital Droplet PCR08:39

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This protocol describes the detailed method of digital droplet PCR (dd-PCR) for precise quantification of circular RNA (circRNA) levels in cells using divergent primers.
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Identification of Circular RNAs using RNA Sequencing08:25

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Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented...
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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli10:38

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

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Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

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Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.
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Related Experiment Video

Updated: Jan 20, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.7K

Methods for analysis of circular RNAs.

Poonam R Pandey1, Rachel Munk1, Gautam Kundu1

  • 1Laboratory of Genetics and Genomics, National Institute on Aging, National Institutes of Health, Baltimore, Maryland.

Wiley Interdisciplinary Reviews. RNA
|September 7, 2019
PubMed
Summary
This summary is machine-generated.

Circular RNAs (circRNAs) are unique RNA molecules found in eukaryotic cells. This review covers methods for studying circRNA identification, function, and challenges in their analysis.

Keywords:
backsplice junctioncircRNAsdivergent primersribonucleoprotein complex

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Quantification of Circular RNAs Using Digital Droplet PCR
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Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing

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Related Experiment Videos

Last Updated: Jan 20, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.7K
Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

4.0K
Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

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Area of Science:

  • RNA Structure and Dynamics
  • RNA Methods

Background:

  • Eukaryotic cells produce numerous circular RNAs (circRNAs) with tissue-specific expression.
  • circRNAs originate from the covalent ligation of 5' and 3' ends of linear precursor RNAs.

Purpose of the Study:

  • To review current resources and molecular methods for circRNA analysis.
  • To discuss challenges and propose alternative approaches for studying circRNAs.

Main Methods:

  • Biochemical and computational approaches adapted for circRNA analysis.
  • Methodologies for quantifying circRNAs, visualizing distribution, and identifying interacting partners.
  • In vitro and in silico RNA analyses.

Main Results:

  • Systematic identification of circRNAs is enabled by circRNA-specific methodologies.
  • Analysis of circRNA biological functions is advancing.
  • Resources and methods for studying circRNAs are continuously evolving.

Conclusions:

  • Adapting traditional RNA analysis methods is crucial for studying circRNAs.
  • Ongoing development of circRNA-specific techniques facilitates deeper understanding of their roles.
  • Addressing analytical challenges is key to unlocking the full potential of circRNA research.