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Dynamic adhesion of immune cells to the vessel wall is a prerequisite for gut homing. Here, we present a protocol for a functional in vitro assay for the impact analysis of anti-integrin antibodies, chemokines or other factors on the dynamic cell adhesion of human cells using addressin-coated...
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Evaluating the Anti-depression Effect of Xiaoyaosan on Chronically-stressed Mice06:08

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Here, we present a protocol to establish the mouse depressive model, observe the behavior changes associated with chronic unpredictable mild stress (CUMS), and evaluate the anti-depression effect of...
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Nanoparticle-Mediated Enhanced Delivery of an Anti-Inflammatory Drug in Microglia-Like Cells01:33

Nanoparticle-Mediated Enhanced Delivery of an Anti-Inflammatory Drug in Microglia-Like Cells

Take cultures of human monocyte-derived microglia-like cells.Introduce nanoparticles containing an anti-inflammatory drug into the experimental culture and the free drug into the control.The nanoparticles feature a hydrophilic shell and a hydrophobic core encapsulating the lipophilic drug.The cells endocytose the nanoparticles, which release the drug intracellularly, enhancing its delivery.The free drug enters by passive diffusion, a process constrained by the concentration gradient, which...
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Vagus Nerve Stimulation Therapy to Manage Treatment-Resistant Depression03:21

Vagus Nerve Stimulation Therapy to Manage Treatment-Resistant Depression

Source: Moeller, S., et al., Vagus Nerve Stimulation As an Adjunctive Neurostimulation Tool in Treatment-resistant Depression. J. Vis. Exp. (2019)This video demonstrates a clinical protocol for Vagus Nerve Stimulation (VNS) as an adjunctive neurostimulation therapy for treatment-resistant depression...
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The Forced Swim Test as a Model of Depressive-like Behavior05:42

The Forced Swim Test as a Model of Depressive-like Behavior

This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral...
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How to Use the H1 Deep Transcranial Magnetic Stimulation Coil for Conditions Other than Depression07:00

How to Use the H1 Deep Transcranial Magnetic Stimulation Coil for Conditions Other than Depression

The H1 deep transcranial magnetic stimulation coil is FDA-cleared for the treatment of depression. We demonstrate how to utilize the H1 for other conditions, such as auditory hallucinations and PTSD, by moving the helmet to different locations over the subject's...
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