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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment...
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Measuring Light-Switching Behavior Using an Occupancy and Light Data Logger05:50

Measuring Light-Switching Behavior Using an Occupancy and Light Data Logger

This article describes a procedure for using and deploying an occupancy and light data logger which allows collecting data about light-switching behavior of participants in field...
6.2K
Measuring Cerebral Blood Volume in the Auditory Cortex Using Vascular Space Occupancy01:21

Measuring Cerebral Blood Volume in the Auditory Cortex Using Vascular Space Occupancy

Position a human participant’s head within the head coil of a functional magnetic resonance imaging, or fMRI, system.Employ vascular space occupancy or VASO, a technique to measure changes in cerebral blood volume, or CBV, the blood volume within the brain.Begin by applying an inversion recovery pulse to suppress signals from blood, allowing only signals from the surrounding tissue within the region to be detected.Next, use MRI-compatible earbuds to deliver auditory stimuli.The stimuli...
161
Cognitive Function and Upper Limb Rehabilitation Training Post-Stroke Using a Digital Occupational Training System07:35

Cognitive Function and Upper Limb Rehabilitation Training Post-Stroke Using a Digital Occupational Training System

The current protocol outlines how the VR-based digital occupational training system enhances the rehabilitation of patients with cognitive impairment and upper limb dysfunction following a...
1.9K
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.
9.3K
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA...
28.5K