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Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis08:15

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

These protocols will help users probe mitochondrial energy metabolism in 3D cancer cell-line-derived spheroids using Seahorse extracellular flux analysis.
3.6K
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism06:04

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study...
11.7K
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila08:22

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila

Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.
9.9K
A Model for Epilepsy of Infectious Etiology using Theiler's Murine Encephalomyelitis Virus05:33

A Model for Epilepsy of Infectious Etiology using Theiler's Murine Encephalomyelitis Virus

Intracerebral infection with the Theiler's murine encephalomyelitis virus (TMEV) in C57BL/6 mice replicates many of the early and chronic clinical symptoms of viral encephalitis and subsequent epilepsy in human patients. This paper describes the virus infection, symptoms, and histopathology of the TMEV model.
3.3K
Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy08:26

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy

Here, we present a protocol for the development and the characterization of a zebrafish model of epilepsy resulting from the transient inhibition of the DEPDC5...
6.2K
Ex Vivo Calcium Imaging for Drosophila Model of Epilepsy04:41

Ex Vivo Calcium Imaging for Drosophila Model of Epilepsy

Here, we present a protocol for ex vivo calcium imaging in GCaMP6-expressing adult Drosophila to monitor epileptiform activities. The protocol provides a valuable tool for investigating ictal events in adult Drosophila through ex vivo calcium imaging, allowing for exploration of the potential mechanisms of epilepsy at the cellular...
2.2K