Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

10.3K
We show how to encode the complex field of laser beams by using a single phase element. A common-path interferometer is employed to mix the phase information displayed into a phase-only spatial light modulator to finally retrieve the desired complex field pattern at the output of an optical imaging...
10.3K
Histochemical Imaging of Cortical Modules in Flattened Brain Sections03:34

Histochemical Imaging of Cortical Modules in Flattened Brain Sections

468
Source: Lauer, S. M., et al. Visualization of Cortical Modules in Flattened Mammalian Cortices. J. Vis. Exp. (2018) This video demonstrates a method to visualize the cortical modules in flattened brain sections using histochemical staining. Pre-fixed sections are treated with a straining reagent that highlights active neural clusters, forming dark, high-contrast regions. After mounting and dehydration, cortical modules appear under a bright-field microscope as...
468
Synaptic Modulation in Drosophila After Exposure to Prolonged Light02:09

Synaptic Modulation in Drosophila After Exposure to Prolonged Light

507
This video demonstrates the rearing of Drosophila melanogaster under specific light conditions to study the role of Bruchpilot proteins in neurotransmitter release and the resulting modulation of synaptic...
507
Quasi-light Storage for Optical Data Packets07:45

Quasi-light Storage for Optical Data Packets

11.3K
The article describes a procedure to store optical data packets with an arbitrary modulation, wavelength, and data rate. These packets are the basis of modern...
11.3K
Characterization of Anisotropic Leaky Mode Modulators for Holovideo09:36

Characterization of Anisotropic Leaky Mode Modulators for Holovideo

8.3K
This work describes fabrication and characterization of anisotropic leaky mode modulators for holographic...
8.3K
Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets07:44

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets

2.5K
Optically activated perfluorocarbon nanodroplets show promise in imaging applications outside of the vascular system. This article will demonstrate how to synthesize these particles, crosslink polyacrylamide phantoms, and modulate the droplets acoustically to enhance their...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving Efficiency and Color Purity of Blue Top-Emitting Organic Light-Emitting Diodes: Distributed Bragg Reflectors for Surface Plasmon Polariton Suppression and Cavity-Resonance Enhancement.

ACS applied materials & interfaces·2026
Same author

Self-filtering monolithic organic/PbS quantum dot photodetector for visible and short-wave infrared selective vision in low-light.

Nature communications·2026
Same author

A Universal Approach to Enhancing Silicon Hot-Carrier Photodetectors for CMOS-Compatible SWIR Imaging.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Event-Driven Neuromorphic Gaze Decoding via e-Skin Electrooculography.

ACS nano·2026
Same author

High-Gain Ag<sub>2</sub>Te/MoS<sub>2</sub> Hybrid Photodetectors for Short-Wave Infrared Imaging.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Simple Process for Flexible Light-Extracting QD Film and White OLED.

Micromachines·2025
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

10.3K

Residual modulation reduction in optical sectioning using a suitable spatial light modulator waveform.

Jeong-Heon Han, Nak-Won Yoo, Myung-Ha Kim

    Applied Optics
    |September 11, 2019
    PubMed
    Summary
    This summary is machine-generated.

    We present a new method for structured illumination microscopy (SIM) using specific illumination patterns to enhance axial response. This technique reduces image artifacts by optimizing spatial frequency and phase-shifting for clearer microscopy images.

    More Related Videos

    Histochemical Imaging of Cortical Modules in Flattened Brain Sections
    03:34

    Histochemical Imaging of Cortical Modules in Flattened Brain Sections

    Published on: May 29, 2025

    468
    Synaptic Modulation in Drosophila After Exposure to Prolonged Light
    02:09

    Synaptic Modulation in Drosophila After Exposure to Prolonged Light

    507

    Related Experiment Videos

    Last Updated: Jan 19, 2026

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.3K
    Histochemical Imaging of Cortical Modules in Flattened Brain Sections
    03:34

    Histochemical Imaging of Cortical Modules in Flattened Brain Sections

    Published on: May 29, 2025

    468
    Synaptic Modulation in Drosophila After Exposure to Prolonged Light
    02:09

    Synaptic Modulation in Drosophila After Exposure to Prolonged Light

    507

    Area of Science:

    • Microscopy
    • Optical Imaging
    • Image Processing

    Background:

    • Structured illumination microscopy (SIM) is a super-resolution technique.
    • Improving the axial response in SIM is crucial for 3D imaging.
    • Residual modulation can degrade image quality in SIM.

    Purpose of the Study:

    • To propose a method for enhancing the axial response of structured illumination microscopy.
    • To investigate the effect of different illumination patterns on residual modulation.
    • To optimize phase-shifting for artifact reduction in SIM.

    Main Methods:

    • Selection of illumination patterns (sinusoidal, square wave) within the imaging system's cutoff frequency.
    • Accurate phase-shifting using electrical spatial light modulator control signals.
    • Analysis of residual modulation reduction based on pattern waveform and spatial frequency.

    Main Results:

    • A reduction in residual modulation was observed with a sinusoidal pattern below the cutoff frequency.
    • The square wave pattern showed a larger reduction in residual modulation as spatial frequency approached one-third of the cutoff frequency.
    • Optimized phase-shifting effectively mitigated residual modulation.

    Conclusions:

    • The proposed method improves the axial response of structured illumination microscopy.
    • Illumination pattern selection and accurate phase-shifting are key to reducing artifacts.
    • This technique offers enhanced image quality for 3D microscopy applications.