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Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope12:54

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Coherent anti-Stokes Raman scattering (CARS) microscopy based on inherent vibration of molecule bonds permits label-free chemically selective live cell imaging. This work presents the implementation of a complementary microscopy technique on a standard multiphoton laser scanning microscope based on a femtosecond Ti:sapphire laser and an OPO...
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A Paper-Based Immunoassay for the Detection of Immunoglobulin G04:48

A Paper-Based Immunoassay for the Detection of Immunoglobulin G

The video demonstrates a paper-based immunoassay, involving amine-functionalized cellulose discs cross-linked with glutaraldehyde. Covalent immobilization of IgG-Fc-specific capture antibodies, followed by the introduction of immunoglobulin G and horseradish peroxidase-conjugated detection antibodies, results in a color change confirming the presence of immunoglobulin...
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Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and...
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Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper05:40

Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper

A protocol for the non-destructive analysis of the fiber content and relative age of...
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Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out07:10

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out

Here, we present a protocol to detect the adulteration of diesel with kerosene using test strips coated with a fluorescent viscosity probe together with a smartphone-based analysis...
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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based...
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