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Related Concept Videos

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Related Experiment Video

Updated: Jan 19, 2026

Focus Ultrasound Based Microbubble Mediated Blood-Brain Barrier Opening: A Technique to Create Localized Transient Openings in Blood-Brain Barrier of Mouse by Sonoporation
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Sonoporation of Cells by a Parallel Stable Cavitation Microbubble Array.

Long Meng1,2, Xiufang Liu1,3, Yuchen Wang1,4

  • 1Paul C. Lauterbur Research Center for Biomedical Imaging Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences 1068 Xueyuan Avenue Shenzhen 518055 China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 12, 2019
PubMed
Summary

This study introduces a microfluidic device for controlled sonoporation using monodispersed microbubbles. This method achieves highly efficient, parallel cell membrane permeabilization for targeted drug delivery.

Keywords:
acoustic radiation forcemembrane permeabilitysonoporationstable cavitationultrasound bioeffects

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Acoustic Technology

Background:

  • Sonoporation utilizes microbubbles and cavitation to create transient cell membrane pores for drug delivery.
  • Current methods face challenges in control and efficiency due to the random nature of microbubble cavitation and broad size distribution.

Purpose of the Study:

  • To develop a microfluidic device for controlled and efficient parallel sonoporation.
  • To investigate the use of monodispersed oscillating microbubbles for homogeneous cell membrane modulation.

Main Methods:

  • A microfluidic device with multi-rectangular channels was designed to generate an array of monodispersed microbubbles.
  • Oscillating microbubbles were characterized using laser Doppler vibrometry to confirm stable cavitation.
  • Acoustic radiation forces were used to trap single cells at microbubble surfaces for targeted sonoporation.

Main Results:

  • Monodispersed microbubbles exhibited stable oscillation with consistent amplitude and resonant frequency.
  • Stable cavitation events were confirmed by detecting harmonic and high harmonic signals.
  • Parallel sonoporation of multiple cells was achieved with high efficiency (96.6 ± 1.74%) at low acoustic pressure (41.7 kPa).

Conclusions:

  • The developed microfluidic device enables controllable and efficient parallel sonoporation.
  • Monodispersed microbubbles and acoustic radiation forces are key to achieving homogeneous cell membrane permeabilization.
  • This technique offers a promising approach for targeted drug delivery applications.