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Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Optimizing Embryo Microinjection for Transgenesis in Drosophila
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Pronuclear Microinjection of One-Cell Embryos.

Melissa A Larson1,2

  • 1Transgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USA. mlarson@kumc.edu.

Methods in Molecular Biology (Clifton, N.J.)
|September 13, 2019
PubMed
Summary
This summary is machine-generated.

Pronuclear microinjection is a key technique for creating genetically modified mice by introducing reagents into zygotes. This method is essential for both traditional transgenesis and advanced gene editing applications.

Keywords:
CRISPRGene editingIn vivoMicroinjectionTransgenicZygotes

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Transgenic and gene-modified mice are crucial models in biological research.
  • Generating these models requires introducing exogenous genetic material into early-stage embryos.
  • The mouse zygote, containing two pronuclei, is the target for these genetic modifications.

Purpose of the Study:

  • To present detailed procedures for pronuclear microinjection in mouse zygotes.
  • To provide a foundational technique applicable to various genetic engineering projects.

Main Methods:

  • Microinjection of DNA constructs into the male pronucleus of mouse zygotes.
  • Application of the technique for generating traditional transgenic mice.
  • Adaptation of the method for introducing gene-editing reagents (ZFNs, TALENs, CRISPR RNAs).

Main Results:

  • Successful introduction of exogenous reagents into mouse zygotes via pronuclear microinjection.
  • Demonstration of the technique's versatility for diverse genetic modification goals.

Conclusions:

  • Pronuclear microinjection is a fundamental and widely applicable technique for creating genetically modified mice.
  • This method facilitates advancements in both transgenesis and precise gene editing in research models.