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Updated: Jan 19, 2026

Mouse In Vivo Placental Targeted CRISPR Manipulation
Published on: April 14, 2023
In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos
Melissa A Larson1,2, Katelin A Gibson1, Jay L Vivian3,4
1Transgenic and Gene-Targeting Institutional Facility, University of Kansas Medical Center, Kansas City, KS, USA.
Abstract:
The CRISPR/Cas9 system has enjoyed enormous success and has now become the standard method of generating gene-modified mouse models. The tools for predicting the activity of CRISPR reagents in the mouse embryo are currently limited and not particularly accurate in predicting if a given reagent will be active. Given the time and cost of generating genetically modified mice, it is highly desirable to use CRISPR reagents that are known to be active in the mouse embryo. In this chapter, we provide a detailed procedure for empirically testing the activity of CRISPR reagents via electroporation into cultured preimplantation mouse embryos. This platform has proven to be rapid, efficient, and applicable to a variety of mouse strains, and can be used for assessing on- and off-target activity through a variety of molecular assays.
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