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Related Concept Videos

Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

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Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
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Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques07:31

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

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Neurons are first characterized electrophysiologically. Then the cytoplasm from the recorded neuron is aspirated and subjected to reverse transcription-PCR analysis to detect the expression of mRNAs for neurotransmitter synthesis enzymes, ion channels, and...
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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

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Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells...
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Non-gated Ion Channels01:24

Non-gated Ion Channels

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Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
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Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies11:42

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

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The reconstitution of the transmembrane protein, KvAP, into giant unilamellar vesicles (GUVs) is demonstrated for two dehydration-rehydration methods — electroformation, and gel-assisted swelling. In both methods, small unilamellar vesicles containing the protein are fused together to form GUVs that can then be studied by fluorescence microscopy and patch-clamp...
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Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

7.6K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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Related Experiment Video

Updated: Jan 19, 2026

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
07:31

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

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Kv2.1 voltage-gated potassium channels in developmental perspective.

Justyna Jędrychowska1,2, Vladimir Korzh1

  • 1International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|September 13, 2019
PubMed
Summary

Kv2.1 channels, crucial for electrical activity, involve Kcnb1 subunits regulating brain development and reproduction. Genetic studies link these channels to neurodevelopmental disorders like epileptic encephalopathy.

Keywords:
Kcnb1Kcng4N- and C-terminalsbrain ventricular systemhydrocephalusmicrocephalyneurodevelopmental diseaseszebrafish

More Related Videos

Voltage-gated Ion Channels
01:26

Voltage-gated Ion Channels

Published on: January 14, 2026

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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

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Related Experiment Videos

Last Updated: Jan 19, 2026

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
07:31

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques

Published on: September 27, 2011

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Voltage-gated Ion Channels
01:26

Voltage-gated Ion Channels

Published on: January 14, 2026

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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Kv2.1 channels are voltage-gated potassium channels composed of electrically-active Kcnb1 α-subunits and regulatory subunits (silent α-subunits or β-subunits).
  • Traditionally, voltage-gated potassium channels were primarily recognized for regulating electrical activity in excitable cells via their transmembrane pore domains.
  • Genetic studies have implicated the pore-forming region of these channels in human neurodevelopmental disorders, including epileptic encephalopathy.

Purpose of the Study:

  • To review the diverse functions of Kv2.1 channels beyond electrical excitability.
  • To explore the roles of Kcnb1-containing channels in brain development and reproduction.
  • To discuss the regulatory interactions between electrically-active and regulatory subunits of Kv2.1 channels.

Main Methods:

  • Literature review of genetic studies, electrophysiology, and animal models.
  • Analysis of N- and C-terminal domain interactions within α-subunits.
  • Examination of regulatory roles of silent α-subunits and β-subunits.

Main Results:

  • Kv2.1 channels, through Kcnb1 subunits, play significant roles in brain development and reproductive functions.
  • Interactions between N- and C-terminal domains of α-subunits form cytoplasmic components regulating potassium channel pores.
  • Regulatory subunits modulate the function of electrically-active subunits, expanding the known roles of Kv2.1 channels.

Conclusions:

  • Kv2.1 channels have multifaceted roles extending to development and reproduction, regulated by subunit interactions.
  • Understanding these regulatory mechanisms is crucial for comprehending neurodevelopmental disorders.
  • This review highlights the complex interplay of subunits in Kv2.1 channel function.