Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture10:08

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture

22.1K
The process of electrospinning polymers for tissue engineering and cell culture is addressed in this article. Specifically, the electrospinning of photoreactive macromers with additional processing capabilities of photopatterning and multi-polymer electrospinning is...
22.1K
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

4.4K
The goal of this protocol is to show the assembly of a biomimetic nanomatrix (NM) with Janus base nanotubes (JBNTs) and fibronectin (FN). When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs...
4.4K
Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

4.0K
This paper lists the steps required to seed neuroblastoma cell lines on previously described three-dimensional collagen-based scaffolds, maintain cell growth for a predetermined timeframe, and retrieve scaffolds for several cell growth and cell behavior analyses and downstream applications, adaptable to satisfy a range of experimental...
4.0K
Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications12:28

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

15.7K
This protocol serves as a comprehensive guideline to fabricate scaffolds via electrospinning with polymer melts in a direct writing mode. We systematically outline the process and define the appropriate parameter settings for achieving targeted scaffold...
15.7K
Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells08:03

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

11.3K
Synthesis and fabrication of electrospun long fibers spanning a larger deposit area via a newly designed collector from a novel biodegradable polymer named poly(glycerol-dodecanoate) (PGD) was reported. The fibers were able to support the growth of cells derived from mouse pluripotent stem...
11.3K
Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold09:37

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

13.4K
Scaffolds capable of fitting within cranio-maxillofacial (CMF) bone defects while exhibiting osteoconductivity and bioactivity are of interest. This protocol describes the preparation of a shape memory scaffold based on polycaprolactone diacrylate (PCL-DA) using a solvent-casting particulate-leaching (SCPL) method employing a fused salt template and application of a bioactive polydopamine...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifunctional Hydrogel-Based Scaffolds: Integrating Conductive Nanomaterials for Smart Wound Healing Applications.

Gels (Basel, Switzerland)·2026
Same author

Bioinspired 3D Printing of Lignocellulose-Based Multimaterial Composites for Extracellular Matrix-Mimicking Architectures.

Biomimetics (Basel, Switzerland)·2026
Same author

A Biomimetic Microfluidic Triple-compartment Periodontium-on-chip for Investigation of Inflammatory Responses in Periodontitis.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

3D-Printed Piezoionic/Bioelectronic Hydrogel for Electro-Metabolic Regulation of Osteogenic Differentiation.

Advanced healthcare materials·2026
Same author

Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose.

Bioactive materials·2026
Same author

3D-printed nanoengineered bioadhesive with tunable stiffness for uncovering electromechanotherapy-assisted cardiac microenvironment.

Biomaterials·2026
Same journal

Correction: Yang et al. Microstructural Characteristics of High-Pressure Die Casting with High Strength-Ductility Synergy Properties: A Review. <i>Materials</i> 2023, <i>16</i>, 1954.

Materials (Basel, Switzerland)·2026
Same journal

Effect of La and Ce Microalloying on the Corrosion Resistance of 0.4Sb Low-Alloy Steel in a Harsh Marine Atmospheric Environment.

Materials (Basel, Switzerland)·2026
Same journal

High-Temperature Properties of Magnesium Ammonium Phosphate Cement Modified with Gold Tailings.

Materials (Basel, Switzerland)·2026
Same journal

A Study on the Evolution of Intermetallic Phase Microstructure and High-Temperature Creep Behavior in Mg-8.0Al-1.0Nd-1.5Gd-Mn Alloys.

Materials (Basel, Switzerland)·2026
Same journal

Material-Driven Clinical Complications in Mechanical Circulatory Support: From Blood-Material Interactions to Device-Related Adverse Events.

Materials (Basel, Switzerland)·2026
Same journal

Influence of Final Irrigation on Calcium Silicate-Based Sealer Dentinal Tubular Penetration: A Systematic Review.

Materials (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
10:08

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture

Published on: October 21, 2009

22.1K

Biomimetic Polymer-Based Engineered Scaffolds for Improved Stem Cell Function.

Dinesh K Patel1, Ki-Taek Lim2

  • 1The Institute of Forest Science, Kangwon National University, Chuncheon-24341, Korea. dineshbhud10@gmail.com.

Materials (Basel, Switzerland)
|September 14, 2019
PubMed
Summary
This summary is machine-generated.

Polymer scaffolds mimic natural tissue environments, supporting stem cell growth and differentiation for tissue engineering. Fabrication methods influence cell response, offering new ways to create extracellular matrix-like materials.

Keywords:
cell functionsextracellular matricesscaffoldsstem cellstissue engineering

More Related Videos

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

4.4K
Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Published on: July 9, 2021

4.0K

Related Experiment Videos

Last Updated: Jan 19, 2026

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
10:08

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture

Published on: October 21, 2009

22.1K
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

4.4K
Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Published on: July 9, 2021

4.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Scaffolds are crucial for tissue engineering due to their physiochemical properties, promoting cell adhesion, proliferation, and differentiation.
  • Stem cells are vital in tissue engineering, with their functions significantly influenced by the extracellular matrix (ECM).
  • Naturally derived ECM contains bioactive motifs that modulate immune responses, highlighting the importance of biomimicry.

Purpose of the Study:

  • To explore the role of polymer scaffolds in tissue engineering.
  • To investigate how scaffold properties and fabrication techniques affect stem cell behavior.
  • To assess the potential of scaffolds in creating ECM-like environments for regenerative applications.

Main Methods:

  • Fabrication of polymer scaffolds using techniques like electrospinning and 3D printing.
  • Evaluation of scaffold properties, including porosity and mechanical strength.
  • Assessment of stem cell adhesion, proliferation, viability, and differentiation on scaffold surfaces.

Main Results:

  • Scaffold porosity and mechanical properties positively influence cell adhesion, proliferation, and differentiation.
  • Different fabrication techniques result in distinct cellular responses on polymer scaffolds.
  • Scaffolds can be engineered to mimic native ECM, regulating cellular functions.

Conclusions:

  • Polymer scaffolds offer a promising platform for tissue engineering by supporting stem cell functions.
  • Tailoring scaffold properties and fabrication methods is key to optimizing regenerative outcomes.
  • Scaffolds provide a viable strategy for generating ECM-like environments, advancing tissue regeneration.