Optical imaging spectroscopy for plant research: more than a colorful picture

Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of cleared plant tissues without physical...

This study presents a method for correlative imaging of drugs in soft tissues using nonlinear optical spectroscopy and mass spectrometry imaging. Combining high-resolution skin imaging with sensitive drug detection offers a valuable approach to study drug distribution in the skin, critical for the development of superior topical...

We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet...

Presented here is a comprehensive protocol to perform ultrafast force-clamp experiments on processive myosin-5 motors, which could be easily extended to the study of other classes of processive motors. The protocol details all the necessary steps, from the setup of the experimental apparatus to sample preparation, data acquisition and...

Source: Laboratories of Judith Danovitch and Nicholaus Noles—University of Louisville
Children are not the best artists. Sometimes it’s easy to pick out the characteristic triangular head, whiskers, and tail of a cat, but children often describe elaborate scenarios that they depict as a beautifully unrecognizable mess. Thus, given children’s questionable artistic talent, how do they know what their drawings, and the drawings of others, represent? One way children identify...

This protocol presents how to live image and analyze the shoot apical meristems from different plant species using laser scanning confocal...