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Updated: Jan 19, 2026

Isolation of Valvular Endothelial Cells
Published on: December 29, 2010
Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles
Nicholas Wanner1, Kewal Asosingh2
1Department of Inflammation and Immunity, Cleveland Clinic, Lerner Research Institute, Cleveland, OH, USA.
Abstract:
Flow-cytometric detection of circulating endothelial cells and endothelial microparticles is an essential tool in studies of vascular diseases. Here we describe the principles and detailed methods for human blood sample processing, storage, labeling, and gating of circulating endothelial elements.
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