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Evaluating the Effect of a Synbiotic Formulation on Alzheimer’s Disease Pathology in Drosophila01:24

Evaluating the Effect of a Synbiotic Formulation on Alzheimer’s Disease Pathology in Drosophila

Take a transgenic Drosophila.The brain of the Drosophila expresses the human amyloid peptide protein or APP and a cleaving enzyme.The enzyme cleaves APP, enabling the formation of beta-amyloid plaque, which leads toAlzheimer's disease or AD pathology in Drosophila.Feed the Drosophila AD model a diet containing a synbiotic formulation.The formulation contains a combination of three bioactive probiotic bacteria and a prebiotic.Inside the fly's gut, the prebiotic supports the growth and survival...
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Mouse Models Of Helicobacter Infection And Gastric Pathologies07:43

Mouse Models Of Helicobacter Infection And Gastric Pathologies

Mice represent an invaluable in vivo model to study infection and diseases caused by gastrointestinal microorganisms. Here, we describe the methods used to study bacterial colonization and histopathological changes in mouse models of Helicobacter pylori-related...
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Symmetric Bihemispheric Postmortem Brain Cutting to Study Healthy and Pathological Brain Conditions in Humans08:29

Symmetric Bihemispheric Postmortem Brain Cutting to Study Healthy and Pathological Brain Conditions in Humans

Organized brain cutting procedures are necessary to correlate specific neuropsychiatric phenomena with definitive neuropathologic diagnoses. Brain cuttings are performed differently based on various clinico-academic contingencies. This protocol describes a symmetric bihemispheric brain cutting procedure to investigate hemispheric differences in human brain pathologies and to maximize current and future biomolecular/neuroimaging...
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How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases13:18

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological...
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DIPLOMA Approach for Standardized Pathology Assessment of Distal Pancreatectomy Specimens10:38

DIPLOMA Approach for Standardized Pathology Assessment of Distal Pancreatectomy Specimens

The current study highlights a standardized approach to the macroscopic assessment of distal pancreatectomy specimens for pancreatic ductal adenocarcinoma, with special emphasis on the measurement of pancreatic dimensions and those of other organs, inking of margins, measurement of tumor size and proximity to margins, lymph node sampling and block...
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Systematic Assessment of Mammalian Skull Specimens for Dental and Temporomandibular Joint Pathology07:26

Systematic Assessment of Mammalian Skull Specimens for Dental and Temporomandibular Joint Pathology

The present protocol describes the techniques for the systematic assessment of skull specimens to characterize anatomical and developmental variations and abnormalities of the teeth, periodontal disease, endodontal disease, and temporomandibular joint...
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