Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
06:11

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

4.2K

ZmILI1 regulates leaf angle by directly affecting liguleless1 expression in maize

Zhenzhen Ren1, Liancheng Wu1, Lixia Ku1

  • 1College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key, Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.

Plant Biotechnology Journal
|September 19, 2019
PubMed
Summary

No abstract available in PubMed .

Keywords:
liguleless1qLA2feedback loopfine mappingleaf anglemaizepromotersignalling pathway

More Related Videos

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

10.0K
Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize06:12

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

2.3K

Related Concept Videos

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging06:11

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Maize leaf primordia are deeply ensheathed and rolled, making them difficult to study. Here, we present methods for preparing transverse sections and unrolled whole mounts of maize leaf primordia for fluorescence and confocal...
4.2K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
10.0K
Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens06:12

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

This manuscript details an optimized inoculation protocol that uses detached maize leaf sheaths for reproducible cytological, physiological, and molecular studies of maize interactions with fungal plant pathogens. The leaf sheaths facilitate real-time observation of cellular interactions between the living plant and fungus in unfixed tissues.
2.3K
Scalable Transfection of Maize Mesophyll Protoplasts08:38

Scalable Transfection of Maize Mesophyll Protoplasts

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll...
3.6K
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein.  Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and...
11.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.7K