Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.4K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.4K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.1K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Mechanisms of Liraglutide Aggregation Induced by Dual Air-Water and Silicone-Oil-Water Interfacial Stress.

Molecular pharmaceutics·2026
Same author

4Pi stimulated Raman scattering for label-free super-resolution chemical imaging.

Science advances·2026
Same author

Effects of biological fixation methods on stimulated Raman scattering microscopy signal.

bioRxiv : the preprint server for biology·2025
Same author

Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform.

Analytical chemistry·2025
Same author

4-Pi Stimulated Raman Scattering for Label-free Super-resolution Chemical Imaging.

bioRxiv : the preprint server for biology·2025
Same author

SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2025
Same journal

The Role of Functional Groups in Substituted Benzoic Acids Used as Dopants in Liquid Crystal Mixtures on the Nematic-Isotropic Transitions.

The journal of physical chemistry. B·2026
Same journal

Hyperfine Coupling Quantifies Hole Delocalization in Triarylamine Radical Cations of D-χ-A Molecules.

The journal of physical chemistry. B·2026
Same journal

A Solvatochromic-Chemometric Framework to Resolve Subtle Polarity Microenvironment Differences in Cycloalkanes Driven by Molecular Conformation and Substituent Effects: A Proof-Of-Concept for Advanced Aviation Fuel Design.

The journal of physical chemistry. B·2026
Same journal

Selective Effects of Backbone Cyclization and Disulfide Bonding as Global Covalent Constraints on the Conformational Ensemble of Sunflower Trypsin Inhibitor-1.

The journal of physical chemistry. B·2026
Same journal

Europium Coordination Structure in Peptide Complexes Resolved with Simulation and X-ray Absorption Spectroscopy.

The journal of physical chemistry. B·2026
Same journal

Competitive Coordination and Structural Evolution of Phenylalanine-Mg<sup>2+</sup> Complexes in Microaqueous Environments: Insights from DFT and Molecular Dynamics Simulations.

The journal of physical chemistry. B·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
10:57

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

3.6K

Frequency Modulation Stimulated Raman Scattering Microscopy through Polarization Encoding.

Andrew H Hill1, Eleanor Munger1, Andrew T Francis1

  • 1Department of Chemistry , University of Washington , Seattle , Washington 98195 , United States.

The Journal of Physical Chemistry. B
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

A new alignment-free frequency modulation stimulated Raman scattering (FM-SRS) microscopy method uses polarization encoding to effectively suppress parasitic background signals. This technique enhances imaging of low-signal molecular distributions and reveals nucleic acid signatures in biological tissues.

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.5K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

165

Related Experiment Videos

Last Updated: Jan 19, 2026

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
10:57

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

3.6K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.5K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

165

Area of Science:

  • Biophotonics
  • Molecular Imaging
  • Spectroscopy

Background:

  • Stimulated Raman scattering (SRS) microscopy images molecular distributions using vibrational contrast.
  • Parasitic background signals in SRS hinder low-signal imaging and quantitative analysis.
  • Existing frequency modulation (FM) methods for background suppression often require multiple images or complex setups.

Purpose of the Study:

  • To develop an alignment-free FM-SRS method for efficient background suppression.
  • To improve the quantitative analysis and sensitivity of SRS microscopy.
  • To enable the detection of low-abundance molecular species in biological samples.

Main Methods:

  • Implemented a novel alignment-free FM-SRS technique utilizing polarization encoding.
  • Employed parabolic amplification of the Stokes pulse for enhanced background removal.
  • Applied the technique to image nucleic acids in murine brain tissue and whole blood.

Main Results:

  • Demonstrated effective suppression of parasitic background signals in SRS microscopy.
  • Successfully unveiled spectral signatures of nucleic acids by suppressing signals from major molecular species.
  • Showcased the technique's capability in complex biological samples like brain tissue and blood.

Conclusions:

  • The developed alignment-free FM-SRS method offers a user-friendly and effective solution for background suppression.
  • This technique significantly enhances the ability to perform quantitative analysis and image low-signal molecular targets.
  • The method is expected to be widely adopted in the SRS microscopy community for diverse biological applications.