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Rapid and room temperature detection of single nucleotide variation with enhanced discrimination by crowding assisted
Miao Cui1, Xiaoyu Zhou2, Xiaoyu Chen3
1Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China. bozheng@cuhk.edu.hk and Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
Chemical Communications (Cambridge, England)
|September 20, 2019
Abstract:
In this paper we report the kinetics based detection of single nucleotide variation (SNV) at room temperature by allele specific extension with different concentrations and types of crowding agents. In general, the crowding conditions enhanced the specificity in the detection of SNV.
