[Effect of prostaglandin E2 on migration of dendritic cells derived from mouse bone marrow]

Objective To explore the effect of prostaglandin E2 (PGE2) on the phenotype and chemotaxis of mouse bone marrow-derived dendritic cells (BMDCs). Methods BMDCs isolated from murine bone marrow and cultured in vitro were divided into 6 groups (0 μg/mL PGE2 group, 1 μg/mL PGE2 group, 5 μg/mL PGE2 group, 0 μg/mL PGE2 plus LPS group, 1 μg/mL PGE2 plus LPS group, 5 μg/mL PGE2 plus LPS group). The expression of surface makers CD40, CD86, major histocompatibility complex II (MHCII), CC chemokine receptor 7 (CCR7) were detected by flow cytometry. The expression of CCR7 protein was detected by Western blot analysis. The migration ability of BMDCs was detected by Transwell^TM assay. The survival rate of BMDCs was detected by CCK-8 assay. Results PGE2 of 1 μg/mL increased the expression of surface molecules on BMDCs and promoted the migration ability of BMDCs. PGE2 of 5 μg/mL reduced the expression of surface molecules on BMDCs and inhibited the migration ability of BMDCs. The change of PGE2 concentration did not affect the survival rate of BMDCs. Conclusion PGE2 was demonstrated a dual regulatory effect on the migration of BMDCs. Low concentration of PGE2 can promote the migration ability of BMDCs, while high concentration of PGE2 shows contrary effect.