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Complete Genome Sequence of Escherichia coli Myophage Mangalitsa

Cameron L Atkison1, Justin Boeckman1,2, Heather Newkirk1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|September 21, 2019
PubMed

Insights

Researchers isolated and annotated the genome of the enteropathogenic Escherichia coli (E. coli) myophage Mangalitsa. This bacteriophage genome analysis aids in understanding E. coli infections and developing novel therapeutic strategies.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriophage research

Background:

  • Enteropathogenic Escherichia coli (E. coli) is a significant human pathogen.
  • Bacteriophages, viruses that infect bacteria, are potential tools for combating bacterial infections.

Purpose of the Study:

  • To isolate and fully annotate the genome of the myophage Mangalitsa, which infects enteropathogenic E. coli.
  • To classify the Mangalitsa myophage based on its genomic features.

Main Methods:

  • Isolation of the myophage Mangalitsa from enteropathogenic E. coli ATCC 23545.
  • Whole-genome sequencing and annotation of the myophage DNA.
  • Comparative analysis of genomic features, including terminal repeats and plaque-forming characteristics.

Main Results:

  • The complete genome sequence of myophage Mangalitsa was determined to be 52,329 base pairs.
  • Genomic analysis revealed predicted terminal repeats.
  • Temperature sensitivity for plaque formation was observed, suggesting a specific classification.

Conclusions:

  • The myophage Mangalitsa genome has been fully characterized.
  • Genomic features suggest Mangalitsa belongs to the unclassified myovirus group.
  • This research contributes to the understanding of bacteriophages targeting enteropathogenic E. coli.

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