Development of Azo-Based Turn-On Chemical Array System for Hydrazine Detection with Fluorescence Pattern Analysis.

Area of Science:

• Analytical Chemistry
• Chemical Sensing
• Fluorescence Spectroscopy

Background:

• Hydrazine is a toxic and reactive chemical requiring sensitive detection methods.
• Existing hydrazine detection methods can be complex or lack selectivity.
• Fluorescence-based sensors offer high sensitivity and potential for visual detection.

Purpose of the Study:

• To develop a facile 'turn-on' chemical sensor array for hydrazine detection.
• To design and synthesize novel fluorogenic probes for hydrazine sensing.
• To establish a fluorescence pattern recognition method for hydrazine quantification.

Main Methods:

• Design and synthesis of four azo-based fluorogenic probes (SF-Azo 01-04).
• Investigation of probe response to hydrazine using fluorescence spectroscopy.
• Development of a cellulose paper-based sensor array system.
• Analysis of fluorescence patterns for hydrazine concentration determination.

Main Results:

• SF-Azo 01-04 probes exhibited significant fluorescence enhancement (up to 800-fold) upon reaction with hydrazine.
• Probes demonstrated excellent selectivity, with minimal interference from various anions and nucleophiles.
• The paper-based array system successfully correlated fluorescence pattern changes with hydrazine concentration.

Conclusions:

• A novel and efficient fluorescence turn-on sensor array for hydrazine detection has been developed.
• The designed fluorogenic probes show high sensitivity and selectivity for hydrazine.
• This method provides a simple and effective platform for on-site hydrazine monitoring.