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Single-cell Microinjection for Cell Communication Analysis
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Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis.

Juanli Yun1, Xiaowei Zheng1, Peng Xu1

  • 1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Small (Weinheim an Der Bergstrasse, Germany)
|October 1, 2019
PubMed
Summary
This summary is machine-generated.

Interfacial nanoinjection (INJ) miniaturizes single-cell assays in nanoliter droplets for high-throughput analysis. Coupled with fluorescence-activated cell sorting (FACS), this method enables diverse applications in cell biology and diagnostics.

Keywords:
dropletfluorescence-activated cell sortingmicrofluidicssingle-cell analysissingle-cell sequencing

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Genomics

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity and identifying rare cells.
  • Existing methods can be complex and require specialized equipment for miniaturization.

Purpose of the Study:

  • To introduce Interfacial Nanoinjection (INJ), a simple method for miniaturizing single-cell assays.
  • To demonstrate the coupling of INJ with Fluorescence-Activated Cell Sorting (FACS) for high-throughput analysis.

Main Methods:

  • Interfacial nanoinjection (INJ) miniaturizes assays into nanoliter water-in-oil droplets.
  • Droplet handler precisely adjusts volumes for multistep reactions with high monodispersity.
  • FACS-INJ pipeline integrates cell sorting with plate well-based single-cell analyses.

Main Results:

  • INJ enables a wide range of single-cell assays within droplets.
  • FACS-INJ facilitates high-throughput analyses including proliferation, drug resistance, PCR, and whole-genome sequencing.
  • The pipeline demonstrates compatibility with diverse samples.

Conclusions:

  • The FACS-INJ pipeline offers a versatile and efficient platform for single-cell analysis.
  • This method has broad applicability in microbiology, cell biology, and biomedical diagnostics.
  • INJ significantly advances the capabilities of single-cell research.