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Scaffold with Micro/Macro-Architecture for Myocardial Alignment Engineering into Complex 3D Cell Patterns
Wanqi Zhang1, Zuyong Wang1, Chao Xie2
1College of Materials Science and Engineering, Hunan University, Changsha, 410082, P. R. China.
Advanced Healthcare Materials
|October 11, 2019
Summary
Engineered scaffolds with micro/macro-architecture guide human mesenchymal stem cell alignment for myocardial tissue engineering. This 3D construct mimics natural heart tissue structure, advancing regenerative medicine possibilities.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Heart function relies on complex tissue structural anisotropy.
- Regenerating aligned heart tissue using macroscale 3D constructs remains challenging in myocardial tissue engineering.
Purpose of the Study:
- To investigate the feasibility of engineered scaffolds with micro/macro-architecture for guiding spatial cell alignment in complex patterns.
- To develop a 3D construct for myocardial tissue engineering that mimics natural heart tissue alignment.
Main Methods:
- Fabrication of stackable dual-structured scaffold layers with linear micro-ridge/groove patterns and macro-through-hole arrays.
- Seeding human mesenchymal stem cells onto the patterned scaffolds and nonpatterned controls.
- Analyzing cell alignment, orientation, and spatial distribution within the scaffold architecture.
Main Results:
- Scaffolds enabled tailorable anisotropy and interconnective free space.
- Human mesenchymal stem cells demonstrated well-organized spreading alignment with precise orientation control on patterned scaffolds compared to controls.
- Observed spatial cell distribution and directional changes in scaffold patterns led to 3D reconstruction of cellular alignment resembling natural myocardial tissue.
Conclusions:
- Micro/macro-architecture engineering is a viable strategy for spatial cell guidance.
- Engineered scaffolds with tailored micro/macro-architecture show potential for biomanufacturing structural alignment in myocardial tissue engineering.
