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Updated: Jan 5, 2026

Human Egg Maturity Assessment and Its Clinical Application
Published on: August 19, 2019
In vitro postovulatory oocyte aging affects H3K9 trimethylation in two-cell embryos after IVF
Theresa Petri1, Debora Dankert1, Hannah Demond2
1Institute of Anatomy, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Background:
The physiological time axis of oocyte maturation comprises highly sensitive processes. A prolonged time span between ovulation and fertilization may impair oocyte developmental competence and subsequent embryo development, possibly due to epigenetic modifications. Since post-translational histone modifications can modify chromatin activity, and trimethylation of H3K9 (H3K9me3) has been shown to increase in the murine oocyte during maturation, here the effect of postovulatory oocyte aging on H3K9me3 was analyzed.
Methods:
The competence of murine oocytes which were aged for 2, 4, 6 and 8 h in vitro after oocyte retrieval to develop to the two-cell and blastocyst stage was determined. Degree of H3K9me3 was analyzed in the postovulatory aged oocytes as well as in the resulting two-cell embryos after IVF.
Results:
The current study shows that postovulatory aging of oocytes for up to eight hours after oocyte retrieval exhibited no effect on two-cell embryo and blastocyst rate; however, changes in H3K9me3 in the resulting two-cell embryos were observed.
Conclusion:
Prolonged postovulatory oocyte aging leads to epigenetic modifications of H3K9. Such modifications may affect the developmental capacity of embryos at post-implantation developmental stages.
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