Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enlargement of the Plasma Membrane

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026

Same author

On the Prediction of pDNA Productivity Across Diverse Bioprocesses Using Ensemble Hybrid Models.

Biotechnology and bioengineering·2026

Same author

High-density perfusion cultures of the marine bacterium Rhodovulum sulfidophilum for the biomanufacturing of oligonucleotides.

Journal of biotechnology·2024

Same author

Soft sensor based on Raman spectroscopy for the in-line monitoring of metabolites and polymer quality in the biomanufacturing of polyhydroxyalkanoates.

Journal of biotechnology·2023

Same author

Role of the gradient slope during the product internal recycling for the multicolumn countercurrent solvent gradient purification of PEGylated proteins.

Journal of chromatography. A·2023

Same author

Glycosylation network mapping and site-specific glycan maturation <i>in vivo</i>.

iScience·2022

Same journal

Cell surface display for nutritional chemicals: Strategies, mechanisms, and evaluation methods.

Biotechnology advances·2026

Same journal

Advancing synthetic biology with engineered chemically inducible gene regulatory systems.

Biotechnology advances·2026

Same journal

Technology-driven revolution in CO<sub>2</sub> fixation: From natural pathways to programmable Biosystems.

Biotechnology advances·2026

Same journal

Enzymes for CO<sub>2</sub> fixation: Discovery, engineering, and applications.

Biotechnology advances·2026

Same journal

Technological advances in extrachromosomal circular DNA detection.

Biotechnology advances·2026

Same journal

Codon compression and novel codon creation for multiplex non-canonical amino acid incorporation.

Biotechnology advances·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 5, 2026

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers

Published on: July 7, 2023

Expansion processes for cell-based therapies.

Ernesto Scibona¹, Massimo Morbidelli¹

¹Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.

Biotechnology Advances

|October 21, 2019

Summary

This summary is machine-generated.

Living cells offer new treatments for chronic diseases, but manufacturing cell therapy products (CTPs) faces challenges. Cell expansion is key to CTP quality and quantity, requiring scalable, cost-efficient methods for clinical use.

Keywords:

Bioreactor Cell culture Cell-based therapies Expansion Scale-up

More Related Videos

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

Published on: December 5, 2025

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jan 5, 2026

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers

Published on: July 7, 2023

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

Efficient and Rapid Generation of CAR-T and Cytokine-Induced Killer Cells in GMP-scalable Devices

Published on: December 5, 2025

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Published on: March 17, 2023

Area of Science:

Biotechnology
Regenerative Medicine
Cell Therapy Manufacturing

Background:

Living cells are promising therapeutic agents for severe chronic diseases.
Cell-based therapies face significant biological, regulatory, economic, and engineering challenges.
Cell expansion is a critical manufacturing step influencing cell therapy product quality and quantity.

Purpose of the Study:

To review current cell culture systems and expansion processes for cell therapy products (CTPs).
To identify challenges in scaling up CTP production.
To highlight the need for scalable, cost-efficient technologies and process optimization.

Main Methods:

Literature review of cell culture systems and expansion processes for CTPs.
Analysis of critical quality attributes determined by cell expansion.
Identification of challenges in translating cell therapies to clinical practice.

Main Results:

Cell expansion significantly impacts CTP number, phenotype, and critical quality attributes.
Current manufacturing processes require optimization for scalability and cost-efficiency.
Bridging the gap between research and commercialization necessitates advanced technologies.

Conclusions:

Scalable and cost-efficient cell expansion technologies are crucial for CTP manufacturing.
Process optimization is essential to overcome challenges in cell therapy production.
Advancements in manufacturing are needed to realize the clinical potential of cell-based therapies.