Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

3.6K
Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.6K
Transduction01:16

Transduction

1.1K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
1.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.1K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide analysis of strand-specific transcription and DNA methylation in <i>Plasmodium falciparum</i> severe malaria.

Frontiers in microbiology·2026
Same author

Unraveling health disparities across cause-specific outcomes associated with emerging air pollutants: Evidence on black carbon and ultrafine particles in Buffalo, New York.

Environmental research·2026
Same author

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026
Same author

LncRNA YIYA drives pancreatic cancer proliferation under high-glucose conditions by reinforcing a RAS-PKM2-dependent Warburg phenotype.

FEBS letters·2026
Same author

Histone H4K5 deacetylation by enhanced HDAC1 expression drives endothelial inflammation in diabetes through repression of the KLF2-eNOS axis.

Human cell·2026
Same author

Cisplatin-Induced Oxidative Stress Regulates YAP to Modulate Epigenome Promoting the Survival of Osteosarcoma Cells.

Biochemistry·2026

Related Experiment Video

Updated: Jan 5, 2026

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
12:28

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Published on: January 11, 2019

7.7K

Drug Tolerant Cells: An Emerging Target With Unique Transcriptomic Features.

Divya Niveditha1, Harshita Sharma1, Anirudha Sahu1

  • 1Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Pilani, India.

Cancer Informatics
|October 23, 2019
PubMed
Summary

Drug-tolerant cancer cells survive chemotherapy, potentially leading to resistance and recurrence. Understanding their unique gene expression is key to developing new treatments that target these cells and prevent tumor regrowth.

Keywords:
OsteosarcomaRNA sequencingdrug resistancedrug-tolerant persisterepigenetic alteration

More Related Videos

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

7.5K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.2K

Related Experiment Videos

Last Updated: Jan 5, 2026

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
12:28

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Published on: January 11, 2019

7.7K
Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

7.5K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

14.2K

Area of Science:

  • Oncology
  • Cancer Biology
  • Molecular Medicine

Background:

  • Cancer therapy outcomes are often compromised by acquired drug resistance.
  • A subpopulation of tumor cells can survive intense drug treatment, contributing to long-term resistance.
  • These drug-tolerant cells are crucial for tumor cell survival and eventual resistance acquisition.

Purpose of the Study:

  • To investigate the transcriptomic characteristics of drug-tolerant osteosarcoma cells.
  • To compare the gene expression profiles of minimally dividing tolerant cells with proliferative persisters and resistant cells.
  • To identify potential therapeutic targets within drug-tolerant cells to prevent tumor recurrence.

Main Methods:

  • Analysis of the transcriptome of osteosarcoma cells surviving high-dose cisplatin treatment.
  • Comparative transcriptomic analysis between drug-tolerant cells, proliferative persisters, and derived resistant cells.

Main Results:

  • Identification of a unique transcriptome specific to drug-tolerant osteosarcoma cells.
  • Characterization of the molecular features distinguishing tolerant cells from more proliferative or resistant populations.
  • Demonstration that drug-tolerant cells exhibit minimal proliferation.

Conclusions:

  • Drug-tolerant cells possess distinct transcriptomic profiles that differ from resistant or sensitive cells.
  • Targeting these specific molecular features of tolerant cells offers a promising strategy to impede tumor recurrence.
  • Understanding drug tolerance mechanisms is critical for improving long-term cancer therapy efficacy.