Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

23.6K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
23.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.3K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ion-Pair Breakers and Anionic Brønsted Acids for Helmholtz-Layer Catalysis with External Electric Fields in Microfluidic Capacitors.

JACS Au·2026
Same author

Flipper dendrimers.

Chemical science·2026
Same author

Densely-functionalized bicyclic cyclopentanones by combined photoinduced 6-<i>endo-trig</i> Giese additions and mild aldol cyclizations.

Organic chemistry frontiers : an international journal of organic chemistry·2026
Same author

Grafting Cell-Penetrating Poly(disulfide)s to Substrates of Interest: Dynamic Covalent Bioconjugation for Traceless Delivery.

Angewandte Chemie (International ed. in English)·2025
Same author

Thiol-mediated uptake of phosphorothioate liposomes, visualized with fluorescent flippers.

Chemical science·2025
Same author

Organocatalytic Microfluidic Double-Layer Capacitors.

Angewandte Chemie (International ed. in English)·2025
Same journal

Symmetry Breaking in Achiral Porphyrins: Noncovalent Origins of Emergent Optical Activity.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Modulation of O<sub>2</sub> Affinity and Enzymatic Activity of Core‒Shell Structured Hemoglobin Nanoparticles.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Stepwise Synthesis of Tetrabenzotriazaporphyrins (TBTAPs) and Their Open 2- and 3-Ring Fragments.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Geometry-Based Neural-Network Prediction of Electron Localization Function Topology in Dense Hydrogen.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Dual Regulation of Charge Carriers Based on Phosphorus-Doped CdS/Nickel Polyphthalocyanine Dyads for Boosting Photocatalytic CO<sub>2</sub> Reduction.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Effects of Biotin on a Fluorescein-Based Photosensitizer Revealed by Multiscale Computational Modeling.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Jan 5, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.9K

Fluorescent Flipper Probes: Comprehensive Twist Coverage.

Karolina Strakova1, Amalia I Poblador-Bahamonde1, Naomi Sakai1

  • 1Department of Organic Chemistry, University of Geneva, Geneva, Switzerland.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|October 23, 2019
PubMed
Summary
This summary is machine-generated.

Researchers optimized "flipper probes" for imaging cell membrane tension. Modifying the probe

Keywords:
chalcogen bondsfluorescent probesforce imagingmechanochemistrymembrane tension

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

940
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.8K

Related Experiment Videos

Last Updated: Jan 5, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.9K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

940
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
12:31

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

23.8K

Area of Science:

  • Biophysical chemistry
  • Cell biology
  • Organic synthesis

Background:

  • Planarizable push-pull probes are essential for imaging membrane tension in living cells.
  • Existing probes based on dithieno[3,2-b:2",3"-d]thiophenes (DTTs) offer a starting point for optimization.

Purpose of the Study:

  • To comprehensively assess the chemical space for optimizing
  • flipper probes
  • to enhance their sensitivity and utility in membrane tension imaging.

Main Methods:

  • Systematic chemical modification of the DTT core structure, focusing on the twist region.
  • Synthesis of novel probe analogues with varying substituents (e.g., methyl, trifluoromethyl).
  • Spectroscopic characterization (excitation/emission maxima, fluorescence intensity, lifetime) and membrane partitioning studies.

Main Results:

  • Probe sensitivity to membrane tension is highly dependent on substituents in the twist region.
  • Removal of methyl groups leads to premature planarization and reduced sensitivity.
  • Incorporation of a trifluoromethyl group induces a significant redshift (>100 nm) in excitation maxima, correlated with membrane order.
  • Optimized probes exhibit record changes in fluorescence properties and enhanced mechanosensitivity.

Conclusions:

  • Chemical modifications, particularly trifluoromethyl substitution, can dramatically enhance the performance of push-pull probes for membrane tension imaging.
  • Understanding the relationship between probe structure, membrane interaction, and photophysical properties is key to designing next-generation mechanosensitive probes.
  • The study reveals kinetic competition between excited states as the origin of fluorescence quantum yields in these probes.