Related Experiment Video
Updated: Jan 5, 2026
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
An immunochromatographic strip sensor for sildenafil and its analogues
Zhongxing Wang1, Xiaoling Wu1, Liqiang Liu1
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. kuangh@jiangnan.edu.cn xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Abstract:
In this study, a new hapten of sildenafil (Sild) was successfully synthesized and a sensitive monoclonal antibody (mAb) against Sild was produced based on this new hapten. The subclass of the mAb was IgG2a, and the half-maximal inhibitory concentration (IC50) of the mAb was 0.53 ng mL-1. Next, an immunochromatographic assay (ICA) was established for detecting Sild and its analogues in functional foods, where the visual detection limit (vLOD) and cut-off values were 0.5 and 20 μg kg-1, respectively. With the aid of a strip scan reader, the ICA can measure Sild with an LOD of 0.7 μg kg-1 and a line range of detection between 1.40 and 13.11 μg kg-1. The whole test process takes only 15 min. Therefore, the ICA provides a useful tool for the on-site detection and rapid initial screening of Sild in functional food samples.
