Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.2K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.2K
Myocarditis II: Clinical Features and Diagnostic Tests01:27

Myocarditis II: Clinical Features and Diagnostic Tests

218
Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...
218
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

744
Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
744

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and initial validation of a brief measure of uncertainty in rheumatic disease.

Journal of health psychology·2026
Same author

Multiorgan involvement and circulating IgG1 predict hypocomplementaemia in IgG4-related disease.

Annals of the rheumatic diseases·2024
Same author

A Severe Case of ANCA-Associated Small Vessel Vasculitis.

Rhode Island medical journal (2013)·2024
Same author

Efficacy and Safety of Inebilizumab in IgG4-Related Disease: Protocol for a Randomized Controlled Trial.

Rheumatology and therapy·2023
Same author

Boosting Awareness of Sclerotic Skin Diseases: Exploring the Inflammatory Overlap of Sclerotic Skin Disease and COVID-19 Vaccination.

Arthritis care & research·2023
Same author

Assessment of patient-reported symptoms and distress in IgG4-related disease (IgG4-RD): Development, clinical validation, and content validation of the IgG4-RD Symptom Severity Index.

Seminars in arthritis and rheumatism·2023

Related Experiment Video

Updated: Jan 4, 2026

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
09:15

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Published on: July 26, 2019

9.8K

Diagnostic Pitfalls in Immunology Testing.

Lewena Maher1, Cory Perugino2

  • 1Internal Medicine, Beth Israel Lahey Health, Burlington, MA, USA.

Clinics in Laboratory Medicine
|November 1, 2019
PubMed
Summary
This summary is machine-generated.

Accurate immunology testing is crucial for diagnosing autoimmune diseases. This review highlights common pitfalls in interpreting rheumatoid factor, antinuclear antibodies, and other key immunology tests to improve diagnostic accuracy.

Keywords:
ANAANCAAntiphospholipidAutoantibodyIgG4Rheumatoid factor

More Related Videos

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.8K
Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate
10:05

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Published on: January 17, 2018

37.0K

Related Experiment Videos

Last Updated: Jan 4, 2026

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
09:15

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Published on: July 26, 2019

9.8K
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.8K
Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate
10:05

Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate

Published on: January 17, 2018

37.0K

Area of Science:

  • Clinical immunology
  • Autoimmune disease diagnostics

Background:

  • Immunology testing is vital for diagnosing autoimmune conditions.
  • Diagnostic accuracy can be compromised by misinterpretation and incomplete understanding of techniques or diseases.
  • Common immunology tests include rheumatoid factor, antinuclear antibodies, antiphospholipid antibodies, antineutrophil cytoplasmic antibodies, and serum IgG4.

Purpose of the Study:

  • To review diagnostic considerations for commonly used immunology tests.
  • To summarize interpretation caveats for specific immunology tests relevant to autoimmune conditions.

Main Methods:

  • Review of diagnostic considerations for commonly used immunology tests.
  • Summarization of interpretation caveats for rheumatoid factor, antinuclear antibodies, antiphospholipid antibodies, antineutrophil cytoplasmic antibodies, and serum IgG4 testing.

Main Results:

  • Diagnostic pitfalls in immunology testing stem from result interpretation and technique/condition understanding.
  • Specific caveats exist for interpreting rheumatoid factor, antinuclear antibodies, antiphospholipid antibodies, antineutrophil cytoplasmic antibodies, and serum IgG4.

Conclusions:

  • Understanding the nuances of specific immunology tests is essential for accurate autoimmune disease diagnosis.
  • Addressing interpretation pitfalls can enhance the clinical utility of immunology testing in autoimmune diagnostics.