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Background-Free Quantitative Surface Enhanced Raman Spectroscopy Analysis Using Core-Shell Nanoparticles with an
Mei Li1,2, Jing-Yu Wang3, Qing-Qi Chen1
1College of Chemistry and Chemical Engineering, State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Energy, College of Materials, Xiamen University, Xiamen 361005, China.
This study introduces a novel Prussian blue (PB) coated gold nanoparticle (Au@PB NPs) method for reliable quantitative Surface-Enhanced Raman Spectroscopy (SERS) analysis. This technique offers an interference-free internal standard for accurate detection in complex samples.
Area of Science:
- Analytical Chemistry
- Materials Science
- Spectroscopy
Background:
- Surface-Enhanced Raman Spectroscopy (SERS) offers ultrasensitive, label-free chemical fingerprinting.
- Reproducibility and stability issues with nanostructured SERS surfaces hinder reliable quantitative analysis.
- Developing robust SERS platforms is crucial for accurate detection in complex matrices.
Purpose of the Study:
- To develop a stable and reproducible SERS platform for quantitative analysis.
- To utilize Prussian blue (PB) as an internal standard for SERS measurements.
- To demonstrate the application of the developed SERS method for detecting dopamine and crystal violet in real-world samples.
Main Methods:
- Coating gold nanoparticles (Au NPs) with ultrathin, uniform Prussian blue (PB) shells to create Au@PB NPs.
- Utilizing the intense and interference-free Raman signal of PB at 2155 cm⁻¹ as an internal standard (IS).
- Testing the stability of Au@PB NPs in acidic solutions and at elevated temperatures (100 °C).
Main Results:
- Au@PB NPs demonstrated excellent stability in strong acid and thermal conditions.
- The PB Raman signal at 2155 cm⁻¹ effectively corrected for SERS intensity fluctuations.
- Successful quantitative detection of dopamine in blood serum and crystal violet in lake water was achieved.
Conclusions:
- The Au@PB NPs strategy provides a convenient, fast, and reliable quantitative SERS technique.
- The integrated internal standard approach enhances accuracy and reproducibility in complex sample analysis.
- This method holds significant potential for diverse applications in analytical chemistry.
