Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transducer Mechanism: Enzyme-Linked Receptors

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

Signal Transduction: Overview

Signal Transduction: Overview

Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026

Same author

Structure-Controlled Molecular Recognition and Charge Transport in Metallized DNA Nanosheets.

Journal of the American Chemical Society·2026

Same author

Author Correction: Atomically precise photothermal nanomachines.

Nature materials·2026

Same author

L-DNA-Based Framework Nucleic Acid Nanodevice for Lysosomal ATP Imaging.

Analytical chemistry·2026

Same author

DNA-Programmed Amorphous PtCu Nanohybrids With Spatially Partitioned Functions for Hydrogen Evolution.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

DNA Framework Nucleator-Enabled Intelligent Hydrogel Interfaces on Living Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026

Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026

Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026

Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026

Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026

Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 4, 2026

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

DNA Framework-Programmed Cell Capture via Topology-Engineered Receptor-Ligand Interactions.

Min Li¹, Hongming Ding², Meihua Lin³

¹Institute of Molecular Medicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine and School of Chemistry and Chemical Engineering , Shanghai Jiao Tong University , Shanghai 200127 , China.

Journal of the American Chemical Society

|November 7, 2019

Summary

This summary is machine-generated.

Researchers engineered cell surface receptor-ligand interactions using tetrahedral DNA frameworks for high-affinity capture of circulating tumor cells (CTCs). This novel approach enhances cancer cell detection and diagnosis.

More Related Videos

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Related Experiment Videos

Last Updated: Jan 4, 2026

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Area of Science:

Biotechnology
Molecular Biology
Nanotechnology

Background:

Receptor-ligand interactions (RLIs) are crucial in biology but complex on cell surfaces due to dynamic receptor distribution.
The traditional keys-and-locks model inadequately describes these complex cell surface RLIs.

Purpose of the Study:

To develop a tetrahedral DNA framework (TDF)-programmed platform for topologically engineering cell surface RLIs.
To enable high-affinity capture of circulating tumor cells (CTCs) by actively recruiting clustered receptors.

Main Methods:

Synthesized n-simplexes using TDFs with 1-3 aptamers targeting epithelial cell adhesion molecule (EpCAM).
Utilized the orthogonal anchoring of ligands on TDF vertices for spatial organization.
Employed 2-simplex probes for CTC capture and evaluated their binding affinity and effect on endocytosis.

Main Results:

The 2-simplex with three EpCAM aptamers demonstrated a ~19-fold increase in binding affinity.
Engineered RLIs prevented cancer cell endocytosis.
Achieved high-efficiency capture of CTCs from clinical breast cancer patient samples.

Conclusions:

TDF-programmed platform offers a powerful method for engineering cell surface RLIs.
This approach facilitates studying RLIs in physiological contexts.
The platform shows significant potential for advancing cancer diagnosis, particularly for CTC detection.