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Updated: Jan 4, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single-cell protein profiling in microchambers with barcoded beads.

Lucas Armbrecht¹, Rafael Sebastian Müller¹, Jonas Nikoloff¹

¹Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, Switzerland.

Microsystems & Nanoengineering

|November 9, 2019

Summary

This summary is machine-generated.

This study introduces a microfluidic platform for multiplexed single-cell protein quantification, overcoming challenges in analyzing individual cell proteins. The method enables precise protein detection, aiding personalized cancer treatment and diverse biological analyses.

Keywords:

Chemistry Engineering

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Last Updated: Jan 4, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: May 23, 2018

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Published on: October 15, 2013

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

Area of Science:

Biotechnology
Molecular Biology
Cancer Research

Background:

Single-cell profiling offers unique insights beyond bulk measurements.
Personalized cancer therapy benefits from individual tumor cell analysis.
Quantifying proteins at the single-cell level remains a significant challenge.

Purpose of the Study:

To develop a microfluidic method for multiplexed single-cell protein quantification.
To enable automated data acquisition and analysis of intracellular or secreted proteins.
To advance personalized cancer treatment through rapid drug efficacy selection.

Main Methods:

A microfluidic platform with 1026 chambers for co-immobilizing single cells and barcoded beads.
Development of on-chip immunoassays for protein detection.
Demonstration using three mammalian cell lines, including breast cancer models.

Main Results:

Multiplexed quantification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), galectin-3 (Gal-3), and galectin-3 binding protein (Gal-3bp) in single cells.
Achieved low detection limits for target proteins (e.g., 1.8 × 10^3 molecules/cell for Gal-3bp).
Successfully differentiated cell types based on Gal-3 and Gal-3bp expression, relevant to metastasis.

Conclusions:

The microfluidic platform facilitates sensitive, multiplexed single-cell protein profiling.
The method is versatile, applicable to various cell types and lipid vesicles.
Commercial barcoded beads allow for easy scaling to hundreds of targets for diverse applications.