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Method to determine the enantiomers of ibuprofen from human urine by high-performance liquid chromatography
D A Nicoll-Griffith1, T Inaba, B K Tang
1Department of Pharmacology, University of Toronto, Ontario, Canada.
Journal of Chromatography
|June 24, 1988
Abstract:
By means of ethyl chloroformate, ibuprofen enantiomers were coupled to 4-methoxyaniline. The resulting amides were resolved from each other and from urinary constituents on a Pirkle column using an isocratic mobile phase with ultraviolet detection at 254 nm. Applicability of this method for the determination of inter-individual differences in urinary metabolic profiles of ibuprofen enantiomers is demonstrated. The chromatographic behavior of the corresponding amide derivatives of two ibuprofen metabolites is also described.