Related Experiment Video For Advanced functional nanomaterials
Updated: Jan 3, 2026
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Nanoparticles characterization using the CAM assay
Soontaree Grace Intasa-Ard1, Albane Birault2
1School of Molecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology, Rayong, Thailand; Institute for Integrated Cell-Material Sciences-Vidyasirimedhi Institute of Science and Technology Research Center, Institute for Advanced Study, Kyoto University, Kyoto, Japan.
Abstract:
The current chapter highlights the use of chorioallantoic membrane (CAM) of fertilized chicken egg for the characterization of nanoparticles applied in cancer nanomedicine. The CAM assay represents a promising alternative to mouse models in term of costs, ease of use, rapidity and ethical issues in particular for the screening of nanoformulations. Hence, the features of nanoparticles including blood retention, biocompatibility, active targeting or tumor accumulation, angiogenic activity, drug delivery and tumor elimination might be simply evaluated via the CAM model. In particular, in this model, embryo organs and morphology, CAM vasculature and blood cells, transplanted tumors on CAM were typically monitored and used for the evaluation of the nanomaterials. With the above advantages, the CAM assay, as highly valuable in vivo model, could be used regularly in pharmaceutical industries.
