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Use of biocompatible redox-active polymers based on carbon nanotubes and modified organic matrices for development of a highly sensitive BOD biosensor.

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A mediator microbial biosensor for assaying general toxicity.

A S Kharkova1, V A Arlyapov1, A D Turovskaya2

  • 1FSBEIHE Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

Enzyme and Microbial Technology
|November 17, 2019
PubMed
Summary
This summary is machine-generated.

A novel biosensor using Paracoccus yeei bacteria and a ferrocene mediator was developed for effective toxicity assessment of cosmetics and perfumes. This innovative approach enhances sensitivity for detecting heavy metals and phenols in real-world samples.

Keywords:
BiosensorMediatorParacoccus yeei bacteriaToxicityVKM B-3302ЕC(50)

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Biosensors offer sensitive detection of environmental contaminants.
  • Assessing toxicity of perfumery and cosmetics requires reliable analytical methods.
  • Optimizing mediator selection is crucial for biosensor performance.

Purpose of the Study:

  • To develop a mediator biosensor using Paracoccus yeei bacteria for toxicity analysis.
  • To propose a method for selecting electron-transport mediators based on kinetic constants.
  • To evaluate the biosensor's efficacy in detecting toxicants in perfumery and cosmetics.

Main Methods:

  • Screening nine potential mediators, focusing on electron transfer and biomaterial interaction constants.
  • Immobilizing the optimal ferrocene mediator in graphite paste.
  • Constructing a biosensor with Paracoccus yeei bacteria and the ferrocene mediator.
  • Testing the biosensor's sensitivity using heavy metals (Cu2+, Zn2+, Pb2+, Cd2+) and phenols (phenol, p-nitrophenol).

Main Results:

  • Ferrocene demonstrated the highest electron transfer efficiency (0.4 ± 0.1 cm/s) and strong interaction with P. yeei (0.023 ± 0.001 dm3/(g·s)).
  • The developed biosensor exhibited enhanced sensitivity to toxicants compared to existing analogues, based on EC50 values.
  • Successful toxicity determination of four perfumery and cosmetics samples was achieved.

Conclusions:

  • The developed ferrocene-mediated biosensor provides a sensitive and effective tool for toxicity assessment.
  • This biosensor shows significant potential for real-time monitoring of toxic substances in consumer products.
  • The proposed mediator selection approach aids in designing high-performance biosensing systems.