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Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Updated: Jan 3, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

Assuring Clonality on the Beacon Digital Cell Line Development Platform.

Kim Le¹, Christopher Tan¹, Huong Le¹

¹Drug Substance Technologies, Process Development, Amgen, Inc., One Amgen Center Drive, Thousand Oaks, CA, 91320, USA.

Biotechnology Journal

|November 20, 2019

Summary

This summary is machine-generated.

The Beacon platform efficiently generates high-assurance single-cell derived production cell lines. This method ensures >99% clonal origin with detailed tracking and minimal resources, overcoming biomanufacturing bottlenecks.

Keywords:

Berkeley Lights Chinese hamster ovary cells cell line development clonality assurance digital cell culture

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Clonogenic Assay: Adherent Cells

Clonogenic Assay: Adherent Cells

Published on: March 13, 2011

Area of Science:

Biotechnology
Cell Line Development
Bioprocess Engineering

Background:

Cell line development for biomanufacturing is often resource- and time-intensive.
Ensuring clonal derivation for single-cell subclones presents significant bottlenecks.
High-throughput technologies are crucial for overcoming these limitations.

Purpose of the Study:

To describe and demonstrate a methodology using the Beacon platform for high-assurance single-cell cloning.
To evaluate the Beacon platform's efficiency in generating production cell lines.
To establish a stringent quality control strategy for verifying clonal origin.

Main Methods:

Utilized the Beacon instrument for culturing, manipulating, and characterizing cells on nanofluidic chips.
Implemented software-controlled operations for automated cell handling.
Developed a stringent in-process quality control strategy for clonal verification.
Validated the workflow using Chinese hamster ovary (CHO) cells expressing fluorescent proteins.

Main Results:

The Beacon platform efficiently generated production cell lines with a superior clonality data package.
Detailed tracking and minimal resource utilization were achieved.
A stringent in-process quality control strategy enabled rapid verification of clonal origin.
Achieved >99% assurance of clonal origin, comparable to existing methods.

Conclusions:

The Beacon platform offers an efficient strategy to ensure high levels of clonality in cell line development.
This methodology provides high assurance of a single-cell progenitor, overcoming previous limitations.
The platform enables rapid verification of clonal origin with detailed tracking and minimal resources.